DETECTION OF ERWINIA CHRYSANTHEMI PV DIANTHICOLA (ECH) BY THE DAS-ELISA METHOD IN SYMPTOMLESS CARNATION CUTTINGS [French]

摘要

This method, using a polyclonal antiserum, the specificity of which was evaluated in this study, can detect up to 5 x 10(3) cfu.mL(-1). Different incubation conditions of carnation stem samples to be tested by DAS-ELISA were studied in order to amplify the primary inoculum in weakly-infected symptomless samples. The aim was to increase the detection limits. The media LB and CVP, which ensure enrichment of Ech in samples, interfere with efficient antigen-antibody interaction. By contrast, incubation of samples in saline at 30 degrees C allows amplification without loss of antibody recognition. The exploitation of this method for routine analysis of carnation stem samples is discussed.