INFLUENCE OF EXTRACTION MEDIUM ON BOIS NOIR AND FLAVESCENCE DOREE DETECTION, USING POLYCLONAL AND MONOCLONAL ANTIBODIES AGAINST STOLBUR AND FLAVESCENCE DOREE PHYTOPLASMAS [French]

摘要

A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) led us to show that a monoclonal antibody, specific for stolbur phytoplasma, recognized a 'Bois noir'-associated agent in extracts of diseased grapevines from different countries (France, Italy and Switzerland). In addition, we developed a common extraction procedure for the ELISA identification of both stolbur and Flavescence doree phytoplasmas.