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Adapting Selected Nucleic Acid Ligands (Aptamers) to Biosensors

机译:使选定的核酸配体(适体)适应生物传感器

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A flexible biosensor has been developed that utilizes immobilized nucleic acid aptamers to specifically detect free nonlabeled non-nucleic acid targets such as proteins. In a model system, an anti-thrombin DNA aptamer was fluorescently labeled and covalently attached to a glass support. Thrombin in solution was selectively detected by following changes in the evanescent-wave-induced fluorescence anisotropy of the immobilized aptamer. The new biosensor can detect as little as 0.7 amol of thrombin in a 140-pL interrogated volume, has a dynamic range of 3 orders of magnitude, has an inter-sensing-element measurement precision of better than 4% RSD over the range 0-200 nM, and requires less than 10 min for sample analysis. The aptamer-sensor format is generalizable and should allow sensitive, selective, and fast deter-mination of a wide range of analytes.
机译:已经开发出一种灵活的生物传感器,该传感器利用固定的核酸适体来特异性检测游离的未标记的非核酸靶标,例如蛋白质。在模型系统中,将抗凝血酶DNA适体进行荧光标记并共价连接至玻璃支持物。通过跟踪e逝波诱导的固定适体荧光各向异性的变化,选择性检测溶液中的凝血酶。新型生物传感器在140 pL的检测体积中可检测到低至0.7 amol的凝血酶,动态范围为3个数量级,感测元件在0-量程内的测量精度优于4%RSD 200 nM,且样品分析时间少于10分钟。适体-传感器格式是可推广的,应允许对各种分析物进行灵敏,选择性和快速的测定。

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