Robert Austin and colleagues at Princeton University have developed a nanofluidic device for DNA restriction mapping.Single strands of DNA were cut by restriction enzymes and analysed within 1 h using the new device.To fabricate the device,the resitarchnrs pattern two paiallfil microchnnnnls on quartz wafers Nanochannels are then formed between the microchanniils A flunrescently labeled DNA strand,a restriction enzyme,and EDTA am added to the loading nucrouhdii-no.,and Mr)"and the restriction enzyme am added to the ext miciocliannel.
展开▼
