Egg yolk oils exert anti-inflammatory effect via regulating Nrf2/ NF-?B pathway

摘要

Ethnopharmacological relevance: Egg yolk oils (EYO) is a traditional Chinese medicine obtained from Gallus gallus domesticus Brisson, which has been used to treat inflammatory related diseases such as cheilitis, ulceration and acute anal fissure. However, the detailed anti-inflammatory mechanism of EYO is still unknown. Aim of the study: The anti-inflammatory activity and mechanism of EYO were investigated in tumor necrosis factor (TNF)-? induced Caco-2 cells. Materials and methods: EYO was obtained by direct-heat extraction (HE), ethanol extraction (EE) and petroleum ether extraction (PE), respectively. Fatty acid compositions of three EYO were measured by gas chromatography (GC). Cell viability, enzyme-linked immunosorbent assay (ELISA), transcriptome, RT-PCR and Western blotting were also performed. Results: Fatty acid compositions of three EYO were different with varied extraction methods. EYO significantly reduced interleukin (IL)-8 secretion. EYO exerted anti-inflammatory effect via coordinating regulation of Nrf2/ NF-?B pathways based on the results of transcriptome, Q-PCR and Western blotting. In detail, PE and HE inhibited the NF-?B pathway, whereas EE exerted anti-inflammatory activity via the Nrf2/NF-?B pathways. Conclusions: The aforementioned results showed the anti-inflammatory mechanism of EYO. These findings might be beneficial to clinical applications of EYO.