Ciprofloxacin and norfloxacin spectrophotometric determination in a fully automated multi-pumping flow system

摘要

A flow-based methodology for the spectrophotometric determination of ciprofloxacin and norfloxacin, based on the oxidation with N-bromosuccinimide in acidic medium, was developed. The proposed procedure was implemented in a multi-pumping flow system, which provided excellent mixing conditions due to the pulsed flow produced by solenoid micro-pumps actuation, resulting on a sensitive, simple, fast, and versatile analytical method. Linear calibration plots were obtained for ciprofloxacin and norfloxacin concentrations ranging from 5 to 70 mgL_~1 with an R.S.D<2.2% (n=10). Detection limits (3σ) were 0.27 mgL_~1 and 0.99mg L_~1 for norfloxacin and ciprofloxacin, respectively.