An Investigation of Liquid Carryover and Sample Residual for a High-Throughput Flow Cytometer Sample Delivery System

摘要

The phenomenon of intersample contamination in air-segmented continuous-flow assays has been studied for many years,and new uses are being found for these sampling techniques every day.One application that has been developed recently employs a flow cytometer to conduct high-throughput screening assays of biological compounds.We have explored the sources of intersample contamination in the system and shown how methods developed previously can be applied to describe these phenomena.Using a simple model,we were able to accurately measure liquid film thickness in the sample tubing and demonstrate the effects of intersample contamination in a flow cytometer assay.Also,measures have been taken to reduce the level of intersample contamination in cytometric screening assays,helping to make the system a more viable tool for drug screening applications.