Rapid size-exclusion high performance liquid chromatography method for the quality control of amyloid-beta oligomers

摘要

Amyloid-beta(A beta) dysmetabolism is thought to be the main trigger for neurodegenerative events in Alzheimer's disease (AD). In particular, soluble A beta oligomers (A beta Os) are proposed as key mediators of synaptic and cognitive dysfunction in AD. Over the past few decades, A beta Os prepared from synthetic A beta have been widely applied in vitro and in vivo, the so-called chemical models of AD, uncovering their multiple neurotoxic mechanisms. However, the lack of a reliable quality control (QC) for synthetic A beta Os may reflect poor experimental reproducibility. In keeping with this, we optimized and validated a rapid and reproducible SEC-HPLC method using fluorescence detection for the QC of synthetic A beta Os. Our analytical method offers an unprecedent alternative to improve the reproducibility of AD chemical models. (C) 2021 Elsevier B.V. All rights reserved.