Natural compounds fromLeea macrophyllaenhance phagocytosis and promoteosteoblasts differentiation byalkaline phosphatase, type 1 collagen, and osteocalcin gene expression

摘要

The current study investigated the immunomodulating and osteoblast differentiation potential of the natural compounds fromLeea macrophylla(LMN). Immunomodulatory effects have been investigated by the phagocytosis ofCandida albicansusing polymorphonuclear neutrophil cells in the in vitro slide method. A bioactivity-guided fractionation technique was used to evaluate the stimulating effect ofL. macrophyllamethanol extract on osteoblast differentiation using mouse osteoblastic cells. A low dose of LMN was found to stimulate the phagocytic effect better than a higher dose. The natural compounds fromL. macrophyllahave significantly increased alkaline phosphatase (ALP) and osteocalcin activities. The LMN promoted the osteoblast differentiation through upregulation of ALP, osteocalcin, and type 1 collagen in a dose-dependent manner. These natural compounds also upregulated ALP, osteocalcin, and type 1 collagen gene expressions. The data suggest that LMN has potential anabolic sequel on bone formation and osteoblast differentiation.