Anodic Oxidation of alpha-Lipoic Acid at a Glassy Carbon Electrode and Its Determination in Dietary Supplements

摘要

Direct electrochemistry of alpha-lipoic acid (ALA) was performed at a glassy carbon electrode using cyclic, differential pulse and square wave voltammetry over a wide range of pH. The oxidation of ALA is an irreversible process, pH independent, and involves the charge transfer of one electron. The diffusion coefficient of ALA was calculated from the results obtained at pH 6.9 in 0.1 M phosphate buffer and was shown to be D_0 = 1.1 x 10~(-5) cm~2 s~(-1). The limits of detection (LOD) and quantification (LOQ) calculated from the results obtained at this pH are 1.8 and 6.1 mu M, respectively.