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The Successful Use of Cellulose Acetate Membrane for Very Low Density Lipoprotein Isolation and Cholesterol Quantitation

机译:醋酸纤维素膜成功用于极低密度脂蛋白的分离和胆固醇定量

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摘要

The lipoproteins have been successfully isolated electrophoretically from the blood samples of patients having suffered from hyperlipoproteinemia type III but having survived myocardial infarction.The VLDL fraction from the cellulose acetate membrane is cut out and the total cholesterol and cholesterol of the VLDL extracted.The cholesterol is determined using an immobilized cholesterol esterase/ oxidase column using a flow injection analysis system.
机译:已成功地通过电泳从患有III型高脂蛋白血症但在心肌梗死中幸存的患者的血样中电泳分离了脂蛋白,从醋酸纤维素膜上切下了VLDL组分,提取了总胆固醇和VLDL中的胆固醇。使用流动注射分析系统,使用固定的胆固醇酯酶/氧化酶色谱柱测定。

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