DETERMINATION OF LISTERIA MONOCYTOGENES IN MILK SAMPLES BY SIGNAL AMPLIFICATION QUARTZ CRYSTAL MICROBALANCE SENSOR

摘要

A piezoelectric crystal biosensor method has been established for detection of listeria monocytogenes (LM) by gold nanoparticles (GNPs) signal amplification. Based on specific recognition of single-stranded DNA (ssDNA) hybridization, a sandwich format of ssDNA probes/complimentary ssDNA probes modified by GNPs/ssDNA of LM was fabricated. This novel strategy allows the detection limit of LM as low as 10 cfu/mL. The biosensor showed friendly biocompatibility, good specificity, acceptable stability, which provided a potential alternative tool for detection of LM in real samples. Furthermore, the developed method could be easily extended to other gene analysis schemes.