DETERMINATION OF POLYETHYLENE GLYCOL MONOESTER ACRYLATE AND POLYETHYLENE GLYCOL DIESTER ACRYLATE USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

摘要

A procedure was developed for the determination of polyethylene glycol monoester acrylate (PEGMA) and polyethylene glycol diester acrylate (PEGDA) by reversed-phase high performance liquid chromatographic (RP-HPLC) with UV detector. Sample was well separated on an SinoChrom ODS-BP (C-18) column (200-4.6mm i.d., 5 lm) with mobile phases composed of acetonitrile-phosphate buffer solution (0.05 mol L1 pH=6.86) in the ratio of 42:58 (v/v). The PEGMA and PEGDA were detected by UV detector at 205 nm, and quantitatively analyzed with an external standard of methyl acrylate. For PEGMA, the linear response ranged from 0.40-105 mol L1 to 2.00- 103mol L1 (r2>0.999), the detection limit was 0.12-105 mol L1, the recovery rate was found to be 93.4%–99.7%. For PEGDA, the linear response ranged from 0.20- 105 mol L1 to 1.00-103mol L1 (r2>0.999), the detection limit was 0.04- 105 mol L1, the recovery rate was found to be 99.1%105.8%. This quantitative method can also be used in the HPLC analysis of other a,b-unsaturated esters.