Comparative Characteristics of the Reaction of Cellular Elements of Human Venous Blood on Contact with Carbon Hemosorbent and Chitosan Fibers in vitro

摘要

The aim of the work was a comparative description of the reaction of cellular elements of venous blood of a person in vitro upon contact with the carbon sorbent used in the small-volume hemoperfusion (SVH) procedure and chitosan fibers. The formation of chitosan fibers was carried out from a 4% solution of chitosan in a 2% solution of acetic acid in a laboratory setup using the coagulation method. Within 60 min of the experiment in samples of blood in contact with hemosorbents in a rotational mode, 26 parameters were recorded on a SySmex XT 1800i hematology analyzer. The optical density was recorded on a UNICO 280 (S) spectrophotometer at wavelengths corresponding to the absorption maxima of the hemoglobin molecule (414 and 540 nm). An analysis of the results showed that chitosan fibers have greater hemocompatibility and the ability to modify the surface of the fibers with selective agents. Based on the data obtained, it can be concluded that chitosan fibers can be used in the SVH procedure both independently and as a neutral matrix for constructing specific hemoactivators for the practical use of this biopolymer in effective medical treatment regimens.