Effects of latanoprost on the expression of TGF-beta 1 and Wnt/beta-catenin signaling pathway in the choroid of form-deprivation myopia rats

摘要

In this study, we investigated the effect of latanoprost on the expression of TGF-beta 1 and Wnt / beta - Catenin signal pathway in the choroid of form-deprivation myopia model rats. Forty rats were randomly divided into two groups: the control group and the FDM model group. Each group had 20 rats. The FDM model group was established by feeding latanoprost daily for 28 days. 15 rats in each group were used to measure the length of the ocular axis and the level of TGF-beta 1 in choroidal tissue; the remaining 5 rats in each group were used for choroidal fibroblast culture. After modeling, the rats were killed, the length of the ocular axis was measured with a vernier caliper, and the level of TGF-beta 1 protein and mRNA in the choroidal tissue of each group were measured with RT-PCR method. Results showed that compared with the control group, there was a significant difference in the axial length of the FDM model group (P 0.05) in the comparison of GSK3 beta protein in choroidal fibroblasts of rats in each group. TGF-beta 1 and APC protein in FDM group were significantly lower than those in the control group (P 0.05) in the ratio of various indexes protein in FDM + ddk1 group and the comparison of TGF-beta 1 and APC protein in FDM ddk1 group and FDM group The expression of dcl3, p21-gsk3 beta and beta - Catenin decreased significantly (P 0.05). The expression of TGF - beta 1 and APC mRNA in FDM group was significantly lower than that in the control group (P In FDM + ddk1 group, TGF-beta 1 and APC mRNA were significantly lower than those in FDM group (P<0.05), while dcl3, p21-gsk3 beta and beta-Catenin mRNA were significantly higher (P<0.05).