Pickering emulsions stabilized by partially acetylated cellulose nanocrystals for oral administration: oils effect and in vivo toxicity

摘要

Pickering emulsions assembled from biopolymer nanoelements are promising as forms of oral delivery systems. In this study, rod-like partially acetylated cellulose nanocrystals (CNCAc) were used for the preparation of oil-in-water Pickering emulsions using decane (model), olive and liquid paraffin oils. It is concluded that 0.5-1.0 wt% of particles are enough to stabilize emulsions in the presence of CNCAc. Stable emulsions with microdroplets ranging from less than 1 to 5 mu m were prepared. The general trend for the studied emulsions is an increase in dynamic viscosity with increasing NaCl concentration. It has been shown (potentiometric titration in conjunction with 2 pK-modeling) that active centers on the surface of CNCAc particles creating an oil/water interface decreased, and cation sorption/desorption processes were hindered as compared to original particles in sol. A decrease in the lifespan of males and females of Drosophila melanogaster was observed when CNCAc and emulsions were introduced into the nutrient medium. CNCAc hydrosol had a negative effect on the permeability of Drosophila's intestinal barrier in males. However, CNCAc-stabilised o/w emulsions with olive or liquid paraffin oil had a positive effect on the permeability of intestinal barrier in Drosophila's females. No toxic effects were observed in mice when orally administered. The results obtained in the acute toxicity study suggests that acute administration of CNCAc and emulsions did not produce death in 50% of mice at the dose of 2000 mg/kg, internal organs were unchanged.