Evaluation of the Antioxidant and Antidiabetic Potential of the Poly Herbal Formulation: Identification of Bioactive Factors

摘要

Background and Objectives: The present investigation is intended to prepare a PolyHerbal Formulation (PHF) with Piper nigrum (fruits), Terminalia paniculata (bark) and Bauhiniapurpurea (bark) and assess their antioxidant and glucose-lowering effects utilizing in vitro models.Methods: The individual plant methanolic extracts and PHF are exposed to phytochemical examinationand to distinguish the bioactive factors by GC-MS. We assessed the antioxidant properties of individualplant extracts and the PHF by using the DPPH scavenging method, H2O2 scavenging assay,TBARS assay and total antioxidant estimation. Likewise, the anti-diabetic activity was assessed byɑ-amylase and α-glucosidase enzyme inhibition and glucose diffusion inhibitory techniques.Results: We found that PHF contains a high measure of total phenolics, total flavonoids and tannincompared to individual plant extracts. The GC-MS identified the bioactive components. We alsofound that PHF had significantly higher antioxidant and glucose-lowering effects than the individualplant concentrates.Conclusion: In conclusion, it could be reasoned that due to the nearness of antioxidant components,the PHF has good potential in the administration of hyperglycemia, diabetes and the related state ofoxidative stress. This study shows that PHF is superior to individual plant extracts, supporting theconventional PHF concept.