Liquid chromatographic method for determination of free and niosome-entrapped nimodipine in mouse plasma and different tissues

摘要

A rapid HPLC method for the quantification of nimodipine in mouse plasma and tissues has been developed in this study, with simple procedure of sample preparation by one-step protein precipitation. The results of HPLC analysis indicated that linear calibration curves were obtained over the concentration range 0.10 - 10.00 mu g/ml for plasma, 0.10 - 20.00 mu g/g for heart, liver, spleen, kidneys, and brain, and 1.00 - 200.00 mu g/g for lung, respectively. The desirable precision and accuracy were achieved, both intraday and interday for plasma and tissue homogenates. Thus, this newly developed procedure was successfully applicable for determination of nimodipine in mouse plasma and tissues following intravenous administration of free and novel niosome-entrapped nimodipine.