Endoplasmic Reticulum Targeting Reactive Oxygen Species Sensor Based on Dihydrofluorescein: Application of Endoplasmic Reticulum Stress

摘要

Endoplasmic reticulum(ER)has a unique redox environment,which plays critical roles in the organelle's function and in its pathological responses such as ER stress.In this work,we introduce an ER-targeting fluorogenic reactive oxygen species(ROS)chemosensor(ER-Flu)from copper(I)-catalyzed alkyne-azide cycloaddition of 3-propargyl ester of 2',7'-dichlorodihydrofluorescein diacetate and N3-glibenclamide,which were adopted as a fluorogenic ROS sensing module and an ER-targeting module,respectively.Thereby,a series of confocal microscopic experiments of ER-Flu demonstrated that the sensor localizes in ER of the live cells and that ROS are elevated in the cells by ER stress inducers such as thapsigargin,brefeldin A,and tunicamycin.