Automated derivatization instrument: Preparation of alditol acetates for analysis of bacterial carbohydrates using gas chromatography mass spectrometry

摘要

An automated derivatization instrument was developed for preparation of alditol acetates for GC/MS profiling of bacterial carbohydrates. The multistage alditol acetate method is now performed sequentially by a computer-controlled instrument. A series of electrically driven solenoid valves are in-line with a 21-sample manifold. A set of solvent valves controls the input of solvent and/or nitrogen gas to each sample chamber. A set of gas valves controls output to atmosphere or vacuum. Additionally, closure of all valves allows the sample to be sealed in a closed chamber. Temperature is also determined automatically. The availability of this instrument could help popularize the alditol acetate procedure and serve as a prototype for automation of other complex derivatization procedures. [References: 21]