New, sensitive and validated HPLC method for simultaneous estimation of venlafaxine and o-desmethylvenlafaxine using fluorescence detector

摘要

A rapid, sensitive, specific, accurate and precise reverse phase high performance liquid chromatographic method (RP-HPLC) has been developed and validated for the simultaneous estimation of venlafaxine (VEN) and o-desmethylvenlafaxine (ODV) in tablet dosage forms. The column used is Agilent Eclipse XDB-C18 column (150 x 4.6 mm, 5 μm) in low pressure gradient mode with mobile phase containing 0.01 M potassium dihydrogen or-thophosphate: acetonitrile (75:25 v/v; pH 4.5). The mobile phase was pumped at 1.0 ml/min with fluorescence detection at excitation wavelength of 226 nm and emission wavelength of 298 nm. The proposed method has been validated with linear range of 0.1 - 8 μg/mL for both VEN and ODV. The % Bias (Accuracy), intra- batch and inter- batch precision values were within ±2% RSD. Limit of detection of VEN and ODV were found to be 0.002 and 0.005 μg/mL, respectively and limit of quantification of VEN and ODV were found to be 0.007 and 0.015 μg/mL, respectively. Total run time was short being 10 min only. Proposed method has been successfully applied for the quantitative determination of VEN and ODV in tablet dosage forms.