Reversed-Phase High-Performance Liquid Chromatography Combined with On-Line UV Diode Array, FT Infrared, and ~1H Nuclear Magnetic Resonance Spectroscopy and Time-of-Flight Mass Spectrometry: Application to a Mixture of Nonsteroidal Antiinflammatory Drugs

摘要

A Prototype multiply hyphenated system has been applied to the analysis of a mixture of nonsteroidal antiinflammatory drugs separated by reversed-phase HPLC. Characterization of the model NSAIDs was achieved via a combination of diode array UV, ~1H NMR, FT-IR spectroscopy, and time-of-flight mass spectrometry. This combination of spectrometers allowed the collection of UV, ~1H NMR, IR, and mass spectra together with atomic composition data enablight almost complete structural characterization to be performed.