Isotope Dilution Analysis of Se in Human Blood Serum by Using High-Power Nitrogen Microwave-Induced Plasma Mass Spectrometry Coupled with a Hydride Generation Technique

摘要

To establish a method for sensitive, accurate, and precise 'determination of Se in real samples, isotope dilution analysis using high-power nitrogen microwave-induced plasma mass spectrometry (N2 MW-IDMS) was conducted. In this study, freeze-dried human blood serum (Standard Reference Material, NIES No. 4) provided by NIES (National Institute for Environmental Studies) was used as a real sample. The measured isotopes of Se were ↑(78)Se and ↑(80)Se which are the major isotopes of Se. The appropriate amount of a Se spike solution was theoretically calculated by using an error multiplication factor (F) and was confirmed experimentally for the isotope dilution analysis. The mass discrimination effect was corrected for by using a standard Se solution for the measurement of Se isotope ratios in the spiked sample. However, the sensitivity for the detection of Se was not so good and the precision of the determination was not improved (2-3%) by N↓(2) MIP-IDMS with use of the conventional nebulizer. Therefore, a hydride generation system was connected to N↓(2) MIP-IDMS as a sample introduction system (HG-N↓(2) MIP-IDMS) in order to establish a more sensitive detection and a more precise determination of Se. A detection limit (3o) of 10 pg mL↑(-1) could be achieved, and the RSD was less than 1% at the concentration level of 5.0-10.0 ng mL↑(-1) by HG-N↓(2) MIP-IDMS. The analytical results were found to be in a good agreement with those obtained by the standard addition method using conventional Ar ICPMS.