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首页> 外文期刊>Biotechnology and Bioengineering >Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilyticaThermobifida cellulosilytica
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Small cause, large effect: Structural characterization of cutinases from Thermobifida cellulosilyticaThermobifida cellulosilytica

机译：效果小，效果大：来自 Thermobifida cellulosilytica 热量差异的Cutinase

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ABSTRACT <section xml:id="bit26372-sec-0001"> > We have investigated the structures of two native cutinases from Thermobifida cellulosilytica , namely Thc_Cut1 and Thc_Cut2 as well as of two variants, Thc_Cut2_DM (Thc_Cut2_ Arg29Asn_Ala30Val) and Thc_Cut2_TM (Thc_Cut2_Arg19Ser_Arg29Asn_Ala30Val). The four enzymes showed different activities towards the aliphatic polyester poly(lactic acid) (PLLA). The crystal structures of the four enzymes were successfully solved and in combination with Small Angle X‐Ray Scattering (SAXS) the structural features responsible for the selectivity difference were elucidated. Analysis of the crystal structures did not indicate significant conformational differences among the different cutinases. However, the distinctive SAXS scattering data collected from the enzymes in solution indicated a remarkable surface charge difference. The difference in the electrostatic and hydrophobic surface properties could explain potential alternative binding modes of the four cutinases on PLLA explaining their distinct activities. Biotechnol. Bioeng. 2017;114: 2481–2488. ? 2017 Wiley Periodicals, Inc. </section> </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“Bit26372-SEC-0001”> <P>我们研究了来自 Thermobifida cellulosilytica </ i>的两个天然切菜酶的结构，即Thc_cut1和Thc_cut2以及两个变体，Thc_cut2_dm（thc_cut2_ arg29asn_ala30val）和thc_cut2_tm （thc_cut2_arg19ser_arg29asn_ala0val）。该四种酶显示出朝向脂族聚酯聚（乳酸）（PLLA）的不同活性。四种酶的晶体结构成功溶解并与小角度X射线散射（SAXS）组合阐明了负责选择性差异的结构特征。晶体结构的分析并未表明不同的Cukinases之间的显着构象差异。然而，从溶液中从酶中收集的独特萨克斯散射数据表明了显着的表面电荷差异。静电和疏水性表面性质的差异可以解释PLLA上的四个CININASE的潜在替代结合模式，解释其独特的活性。 Biotechnol。生物。 2017; 114：2481-2488。还2017 Wiley期刊，Inc。</ p> </ section> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15017/'>《Biotechnology and Bioengineering》</a> <b style="margin: 0 2px;">|</b><span>2017年第11期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ribitsch Doris&option=202" target="_blank" rel="nofollow">Ribitsch Doris;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hromic Altijana&option=202" target="_blank" rel="nofollow">Hromic Altijana;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zitzenbacher Sabine&option=202" target="_blank" rel="nofollow">Zitzenbacher Sabine;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zartl Barbara&option=202" target="_blank" rel="nofollow">Zartl Barbara;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gamerith Caroline&option=202" target="_blank" rel="nofollow">Gamerith Caroline;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pellis Alessandro&option=202" target="_blank" rel="nofollow">Pellis Alessandro;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jungbauer Alois&option=202" target="_blank" rel="nofollow">Jungbauer Alois;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=?yskowski Andrzej&option=202" target="_blank" rel="nofollow">?yskowski Andrzej;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Steinkellner Georg&option=202" target="_blank" rel="nofollow">Steinkellner Georg;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gruber Karl&option=202" target="_blank" rel="nofollow">Gruber Karl;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tscheliessnig Rupert&option=202" target="_blank" rel="nofollow">Tscheliessnig Rupert;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Herrero Acero Enrique&option=202" target="_blank" rel="nofollow">Herrero Acero Enrique;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guebitz Georg M.&option=202" target="_blank" rel="nofollow">Guebitz Georg M.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Institute of Molecular BiosciencesUniversity of GrazGraz Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Institute of Environmental BiotechnologyUniversity of Natural Resources and Life SciencesVienna Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Institute of Molecular BiosciencesUniversity of GrazGraz Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> <p>Austrian Centre of Industrial Biotechnology ACIBPetergsasse 14 8010 Graz Austria;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme structure&option=203" rel="nofollow">enzyme structure;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=polyester hydrolysis&option=203" rel="nofollow">polyester hydrolysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=poly(lactic acid)&option=203" rel="nofollow">poly(lactic acid);</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cutinases&option=203" rel="nofollow">cutinases;</a> </p> <div class="translation"> 机译：酶结构;聚酯水解;聚（乳酸）;cutinases; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881934.html">Small cause, large effect: Structural characterization of cutinases from <i xmlns="http://www.wiley.com/namespaces/wiley">Thermobifida cellulosilyticaThermobifida cellulosilytica</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ribitsch Doris&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ribitsch Doris,</a> <a 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Ghorbanpour,</a> <span>-1</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：巴斯德毕赤酵母表达的角质酶1从嗜热纤维梭菌的酶降解芳香族和脂肪族聚酯。</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/010007694561.html">Enlarging the tools for efficient enzymatic polycondensation: structural and catalytic features of cutinase 1 from Thermobifida cellulosilytica</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pellis Alessandro&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Pellis Alessandro,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ferrario Valerio&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ferrario Valerio,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zartl Barbara&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zartl Barbara,</a> <span>2016</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：扩大有效酶促缩聚的工具：Thermobifida cellulosilytica角质酶1的结构和催化特性</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130501221193.html">THERMOBIFIDA FUSCA CUTINASE MUTANT AND METHOD FOR SOLUBLE EXPRESSION OF SAME</a> <b>[P]</b> . <span> 外国专利： <!-- --> WO2021248363A1 </span> <span> . 2021-12-16</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：Thermobifida Fusca Cutinase突变体和可溶性表达的方法 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130415563647.html">Method for enhancing extracellular secretion of recombinant proteins in <I>Escherichia coli </I>by co-expressing <I>Thermobifida fusca </I>cutinase</a> <b>[P]</b> . <span> 外国专利： <!-- 美国专利： --> US9109212B2 </span> <span> . 2015-08-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过共表达<I> Thermobifida fusca </ I>角质酶增强大肠杆菌</ i>中重组蛋白的细胞外分泌的方法 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130417172332.html">DEVELOPMENT OF BIOPROCESS FOR PRODUCTION OF NOVEL CUTINASES FROM THERMOBIFIDA FUSCA IN E. COLL BL21 (DE3) ACCESSION NO. MTCC 5725 AND ACCESSION NO. MTCC 5726</a> <b>[P]</b> . <span> 外国专利： <!-- 世界知识产权组织专利： --> WO2014097321A1 </span> <span> . 2014-06-26</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：开发用于在大肠杆菌BL21（DE3）中从毛孔霉菌中产生新的蛋白酶的生物过程MTCC 5725和访问号MTCC 5726] </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028288324','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> 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