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Improved enzymatic performance of graphene‐immobilized β‐glucosidase A in the presence of glucose‐6‐phosphate

机译：在葡萄糖-6-磷酸盐存在下改善石墨烯 - 固定的β-葡糖苷酶A的酶促性能

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Abstract >Optimization of cellulose enzymatic hydrolysis is crucial for cost‐effective bioethanol production from lignocellulosic biomass. Enzyme immobilization in solid support allows enzyme recycling for reuse, lowering hydrolysis costs. Graphene is a nanomaterial isolated in 2004, which possesses exceptional properties for biomolecule immobilization. This study evaluates the potential for β‐glucosidase recycling by immobilization on graphene nanosheets. Data reported here demonstrated that graphene‐immobilized β‐glucosidase can be recycled for at least eight cycles. Immobilization did not change the optimal temperature of catalysis and improved enzymatic stability upon storage. The role of glucose‐6‐phosphate on immobilized enzyme was also investigated, demonstrating that glucose‐6‐phosphate acts as a mixed‐type activator and improves storage stability of immobilized enzyme. Complete cellulose hydrolysis using graphene‐immobilized β‐glucosidase in the presence of glucose‐6‐phosphate resulted in greatly improved hydrolysis rates, demonstrating the potential of this strategy for biomass hydrolysis. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >优化纤维素酶水解对于从木质纤维素生物质的成本有效的生物乙醇生产至关重要。固体酶固定化允许酶回收用于再利用，降低水解成本。石墨烯是2004年纳米材料分离，其具有卓越的生物分子固定性。该研究评估了通过固定在石墨烯纳米片上的β-葡糖苷酶再循环的可能性。这里报道的数据证明了石墨烯 - 固定化β-葡糖苷酶可以再循环至少8个循环。固定化并未改变催化的最佳温度并改善储存时酶促稳定性。研究了葡萄糖-6-磷酸在固定化酶上的作用，证明葡萄糖-6-磷酸盐作为混合型活化剂，提高固定化酶的储存稳定性。在葡萄糖-6-磷酸盐存在下，使用石墨烯固定化β-葡糖苷酶完全水解，导致水解速率大大提高，展示了这种生物质水解策略的潜力。</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2018年第2期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Albino Gomes Anderson&option=202" target="_blank" rel="nofollow">Albino Gomes Anderson;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pazinatto Telli Elisa&option=202" target="_blank" rel="nofollow">Pazinatto Telli Elisa;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Miletti Luiz Claudio&option=202" target="_blank" rel="nofollow">Miletti Luiz Claudio;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Skoronski Everton&option=202" target="_blank" rel="nofollow">Skoronski Everton;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gomes Ghislandi Marcos&option=202" target="_blank" rel="nofollow">Gomes Ghislandi Marcos;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Felippe da Silva Gustavo&option=202" target="_blank" rel="nofollow">Felippe da Silva Gustavo;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Borba Magalh?es Maria de Lourdes&option=202" target="_blank" rel="nofollow">Borba Magalh?es Maria de Lourdes;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Food and Animal ScienceState University of Santa CatarinaLages Brazil;</p> <p>Department of Food and Animal ScienceState University of Santa CatarinaLages Brazil;</p> <p>Department of Food and Animal ScienceState University of Santa CatarinaLages Brazil;</p> <p>Department of Environmental EngineeringState University of Santa CatarinaLages Brazil;</p> <p>Department of Materials EngineeringRural Federal University of PernambucoCabo de Santo Agostinho Brazil;</p> <p>Department of Forest EngineeringState University of Santa CatarinaLages Brazil;</p> <p>Department of Food and Animal ScienceState University of Santa CatarinaLages Brazil;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bacillus polymyxa&option=203" rel="nofollow">Bacillus polymyxa;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=β‐glucosidase A&option=203" rel="nofollow">β‐glucosidase A;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioethanol&option=203" rel="nofollow">bioethanol;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme immobilization&option=203" rel="nofollow">enzyme immobilization;</a> </p> <div class="translation"> 机译：芽孢杆菌;β-葡糖苷酶A;生物乙醇;酶固定化; </div> </li> 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H.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Issacs, S. H.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wilke, C. R.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Wilke, C. R. </a> <span>1978</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：固定化B-葡萄糖苷酶在纤维素酶水解中的应用</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_science-china-information-sciences_thesis/0201258364344.html">A glucose biosensor based on direct electrochemistry of glucose oxidase immobilized onto platinum nanoparticles modified graphene electrode</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=LIU AiRong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . LIU AiRong</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=HUANG ShiMing&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,HUANG ShiMing</a> <span> <a href="/journal-cn-17558/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国科学 </a> </span> <span> . 2012</span><span>,第007期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_academic-abstracts-china-colleges-universities-energy-power-engineering_thesis/0201229093810.html">Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Alireza AHMADIAN YAZDI&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Alireza AHMADIAN YAZDI</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jie XU&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Jie XU</a> <span> <a href="/journal-cn-8420/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国高等学校学术文摘·能源与动力工程 </a> </span> <span> . 2018</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-9858_thesis/020222353830.html">AB-Chord Using Dual Finger Tables to Improve Inquiry Performance</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Guozhi CHEN&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . Guozhi CHEN</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shenggang WAN&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Shenggang WAN</a> <span> <a href="/conference-cn-9858/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2011年青年通信国际会议(ICYC2011) </a> <span> <span> . 2011</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020311468448.html">Retrospective Study of Glucose-6-Phosphate-Dehydrogenase Deficiency</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=高秀梅&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 高秀梅</a> <span> . 2010</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130477637157.html">Immobilized glucose oxidase-catalase preparation and its use in the enzymatic oxidation of glucose</a> <b>[P]</b> . <span> 外国专利：  EP0017708A3 </span> <span> . 1981-09-02</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：固定化葡萄糖氧化酶-过氧化氢酶的制备及其在葡萄糖酶促氧化中的应用 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130478552701.html">INNOVATIVE IMMOBILIZED GLUCOSE OXIDASE CATALASE PREPARATION AND ITS USE FOR ENZYMATIC GLUCOSE OXIDATION</a> <b>[P]</b> . <span> 外国专利：  DE2911192A1 </span> <span> . 1980-10-02</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：创新的固定化葡萄糖氧化酶过氧化氢酶的制备及其在酶法葡萄糖氧化中的应用 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130478933276.html">HIS UNKNOWN IMMOBILIZED GLUCOSE OXIDASE CATALASE PREPARATION AND ITS USE FOR ENZYMATIC GLUCOSE OXIDATION</a> <b>[P]</b> . <span> 外国专利：  DK124080A </span> <span> . 1980-09-23</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：他未知的固定化葡萄糖氧化酶过氧化氢酶的制备及其在酶法葡萄糖氧化中的用途 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028288099','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" 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