Production of 2,3‐butanediol by Klebsiella pneumoniae BLh‐1 and Pantoea agglomerans BL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Cortivo Paulo R. D.; Machado Jonas; Hickert Lilian R.; Rossi Daniele M.; Ayub Marco A. Z.

摘要

Abstract > We investigated the production of 2,3‐butanediol by two enterobacteria isolated from an environmental consortium, <fc> <fi>Klebsiella pneumoniae</fi> </fc> BLh‐1 and Pantoea agglomerans BL1, in a bioprocess using acid and enzymatic hydrolysates of soybean hull as substrates. Cultivations were carried out in orbital shaker under microaerophilic conditions, at 30°C and 37°C, for both bacteria. Both hydrolysates presented high osmotic pressures, around 2,000?mOsm/kg, with varying concentrations of glucose, xylose, and arabinose. Both bacteria were able to grow in the hydrolysates, at both temperatures, and they efficiently converted sugars into 2,3‐butanediol, showing yields varying from 0.25 to 0.51?g/g of sugars and maximum 2,3‐butanediol concentrations varying from 6.4 to 21.9 g/L. Other metabolic products were also obtained in lower amounts, notably ethanol, which peaked at 3.6 g/L in cultures using the enzymatic hydrolysate at 30°C. These results suggest the potential use of these recently isolated bacteria to convert lignocellulosic biomass hydrolysates into value‐added products. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 我们调查了由来自环境联盟分离的两种肠杆菌的2,3-丁二醇的生产， <FC> <fi> klebsiella pneumoniae </ Fi> </ FC> BLH-1和 Pantoea Agglomerans </ i> BL1，在使用酸和酶的酸和酶水解产物作为底物的生物处理。对于两种细菌，在30°C和37℃下，在微苯化的条件下在轨道振荡器下进行培养。两种水解产物呈现出高渗透压，约2,000〜MOSM / kg，具有不同浓度的葡萄糖，木糖和阿拉伯糖。两种细菌能够在两个温度下在水解产物中生长，并且它们将糖有效地转化为2,3-丁二醇，显示出0.25-0.51Ω·克/克的糖和最大2,3-丁二醇浓度从6.4变化的产率。到21.9克/升。其他代谢产物也以较低量，特别是乙醇获得，其在30℃下使用酶水解产物在3.6g / L的培养物中达到3.6g / l。这些结果表明，这些最近分离的细菌的潜在用途将木质纤维素生物质的水解产物转化为增值产物。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cortivo Paulo R. D.&option=202" target="_blank" rel="nofollow">Cortivo Paulo R. D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Machado Jonas&option=202" target="_blank" rel="nofollow">Machado Jonas;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hickert Lilian R.&option=202" target="_blank" rel="nofollow">Hickert Lilian R.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rossi Daniele M.&option=202" target="_blank" rel="nofollow">Rossi Daniele M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ayub Marco A. Z.&option=202" target="_blank" rel="nofollow">Ayub Marco A. Z.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Biotechnology &</p> <p>Biochemical Engineering Laboratory (BiotecLab)Federal University of Rio Grande do SulPorto Alegre Rio Grande do Sul Brazil;</p> <p>Biotechnology &</p> <p>Biochemical Engineering Laboratory (BiotecLab)Federal University of Rio Grande do SulPorto Alegre Rio Grande do Sul Brazil;</p> <p>Biotechnology &</p> <p>Biochemical Engineering Laboratory (BiotecLab)Federal University of Rio Grande do SulPorto Alegre Rio Grande do Sul Brazil;</p> <p>Department of Chemical EngineeringFederal University of Rio Grande do SulPorto Alegre Rio Grande do Sul Brazil;</p> <p>Biotechnology &</p> <p>Biochemical Engineering Laboratory (BiotecLab)Federal University of Rio Grande do SulPorto Alegre Rio Grande do Sul Brazil;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=2&option=203" rel="nofollow">2;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=3‐butanediol&option=203" rel="nofollow">3‐butanediol;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioprocess&option=203" rel="nofollow">bioprocess;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Klebsiella pneumoniae&option=203" rel="nofollow">Klebsiella pneumoniae;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lignocellulosic biomass hydrolysates&option=203" rel="nofollow">lignocellulosic biomass hydrolysates;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pantoea agglomerans&option=203" rel="nofollow">Pantoea agglomerans;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=soybean hull&option=203" rel="nofollow">soybean hull;</a> </p> <div class="translation"> 机译：2;3-丁二醇;生物过程;Klebsiella肺炎;木质纤维素生物量水解酸盐;Pantoea凝聚体;大豆船体; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" 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Saddler </a> <span>1982</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过在乙酸存在下，在高糖浓度上增强2,3-丁二醇的生产产生的2,3-丁二醇</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130416682172.html">Method for Preparing 2,3-butanediol from Commercial carbon source by Transformed Klebsiella pneumoniae</a> <b>[P]</b> . <span> 外国专利：  KR101408304B1 </span> <span> . 2014-06-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：转化肺炎克雷伯菌由商业碳源制备2,3-丁二醇的方法 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130419309693.html">Heterologous Klebsiella pneumoniae strains for producing 2,3-butanediol and manufacturing method</a> <b>[P]</b> . <span> 外国专利：  KR101298988B1 </span> <span> . 2013-08-26</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：用于生产2，3-丁二醇的异源肺炎克雷伯菌菌株及其制备方法 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130416924329.html">METHOD FOR PREPARING 2,3-BUTANEDIOL FROM COMMERCIAL CARBON SOURCE BY TRANSFORMED KLEBSIELLA PNEUMONIAE</a> <b>[P]</b> . <span> 外国专利：  KR20130133616A </span> <span> . 2013-12-09</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：转化的肺炎克雷伯氏菌从商业碳源制备2,3-丁二醇的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> 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