Hybrid coating of alginate microbeads based on protein‐biopolymer multilayers for encapsulation of probiotics

Yucel Falco Cigdem; Amadei Federico; Dhayal Surender K.; Cárdenas Marité; Tanaka Motomu; Risbo Jens

摘要

Abstract > A hybrid coating based on multilayers of proteins and biopolymers was developed to enhance the protection performance of alginate microbeads against acidic conditions for delivery of probiotics ( <fc> <fi>L</fi> </fc> actobacillus <fc> <fi>rhamnosus</fi> </fc> GG). Zeta potential measurements and quartz crystal microbalance with dissipation confirmed layer‐by‐layer deposition of protein‐polymer layers. The stability of protein‐based coatings during simulated gastric fluid (SGF) treatment was monitored by microscopy. Protein‐coated microbeads were partially dismantled, whereas polymer‐coated microbeads were intact after a sequential treatment in simulated gastric and intestinal fluids. This suggests that hybrid formulation offers an advantage over the coatings based on biopolymer multilayers in terms of better release of bacteria. Uncoated alginate microbeads completely dissolved and could not protect bacteria after SGF treatment whereas microbeads with hybrid coating showed increased physical stability and a modest decrease of culturability of 3.8 log units. Therefore, this work provides a concept for future protein‐based hybrid coatings for bacterial delivery systems. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 开发了一种基于多层蛋白质和生物聚合物的杂化涂层，增强了藻酸盐微珠的保护性能，免于酸性益生菌的酸性条件（ <FC> <fi> l </ fi> </ FC> actobacillus </ i> <FC> <fi> rhamosus </ fi> </ FC> GG）。 Zeta电位测量和石英晶体微稳态，耗散证实蛋白质聚合物层的逐层沉积。通过显微镜监测模拟胃液（SGF）处理期间蛋白质的涂层的稳定性。部分拆除蛋白质涂覆的微珠，而在模拟胃和肠液中连续治疗后，聚合物涂覆的微珠完整。这表明混合制剂在基于生物聚合物多层的涂覆方面提供了优于涂层的优势，从而更好地释放细菌。未涂层的海藻酸盐微珠完全溶解，并且在SGF处理后无法保护细菌，而具有杂化涂层的微珠显示出具有增加的物理稳定性和3.8对数单位的培养性的适度降低。因此，这项工作为细菌输送系统提供了未来蛋白质的杂合涂层的概念。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yucel Falco Cigdem&option=202" target="_blank" rel="nofollow">Yucel Falco Cigdem;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Amadei Federico&option=202" target="_blank" rel="nofollow">Amadei Federico;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dhayal Surender K.&option=202" target="_blank" rel="nofollow">Dhayal Surender K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cárdenas Marité&option=202" target="_blank" rel="nofollow">Cárdenas Marité;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tanaka Motomu&option=202" target="_blank" rel="nofollow">Tanaka Motomu;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Risbo Jens&option=202" target="_blank" rel="nofollow">Risbo Jens;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>University of CopenhagenDepartment of Food ScienceCopenhagen Denmark;</p> <p>Heidelberg University Institute for Physical ChemistryHeidelberg Germany;</p> <p>Research &</p> <p>Development Chr. Hansen A/SHoersholm Denmark;</p> <p>Biomedical Laboratory Science and Biofilm Research Center for Biointerfaces Faculty of Health and SocietyMalm? UniversityMalm? Sweden;</p> <p>Heidelberg University Institute for Physical ChemistryHeidelberg Germany;</p> <p>University of CopenhagenDepartment of Food ScienceCopenhagen Denmark;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=alginate&option=203" rel="nofollow">alginate;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=chitosan&option=203" rel="nofollow">chitosan;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=lactoferrin&option=203" rel="nofollow">lactoferrin;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=layer‐by‐layer&option=203" rel="nofollow">layer‐by‐layer;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=microencapsulation of probiotics&option=203" rel="nofollow">microencapsulation of probiotics;</a> </p> <div class="translation"> 机译：藻酸盐;壳聚糖;乳蛋白;逐层;益生菌的微胶囊; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_nano-express-english-version_thesis/0201259585262.html">Upconversion Nanoparticles-Encoded Hydrogel Microbeads-Based Multiplexed Protein Detection</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Swati Shikha&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Swati Shikha,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Xiang Zheng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Xiang Zheng,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yong Zhang&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Yong Zhang </a> <a href="/journal-foreign-18435/" target="_blank" rel="nofollow" class="tuijian_authcolor">纳微快报：英文版 .</a> <span>2018</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-physics-b_thesis/0201251224669.html">Modified-DBR-based semi-omnidirectional multilayer anti-reflection coating for tandem solar cells</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ali Bahrami&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Ali Bahrami,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shahram Mohammadnejad&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Shahram Mohammadnejad,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Nima Jouyandeh Abkenar&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Nima Jouyandeh Abkenar </a> <a href="/journal-foreign-4764/" target="_blank" rel="nofollow" class="tuijian_authcolor">中国物理：英文版 .</a> <span>2014</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/070404244385.html">Survivability of probiotics encapsulated in alginate gel microbeads using a novel impinging aerosols method.</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sohail A&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sohail A,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Turner M. 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Arturo Talledo1</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Junior Asencios1&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Junior Asencios1</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Karin Paucar1&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Karin Paucar1</a> <span> <a href="/journal-cn-54327/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 材料科学与工程：中英文A版 </a> </span> <span> . 2015</span><span>,第007期</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130420168835.html">Preparation of probiotic bacteria for oral administration to farmed fish based on encapsulation in alginate hydrogels.</a> <b>[P]</b> . <span> 外国专利：  ES2390428B1 </span> <span> . 2013-10-01</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于封装在藻酸盐水凝胶中的益生菌，用于对养殖鱼类口服给药。 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130420168836.html">Preparation of probiotic bacteria for oral administration to farmed fish based on encapsulation in alginate hydrogels.</a> <b>[P]</b> . <span> 外国专利：  ES2390428A1 </span> <span> . 2012-11-13</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基于封装在藻酸盐水凝胶中的益生菌，用于对养殖鱼类口服给药。 </span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130442493732.html">Process for encapsulation of Marine Bacteria probiotic Live probiotic strains in an Alginate Matrix and / or carrageenan that remain viable for long periods of time and PERmite keep them available for use in a simple way.</a> <b>[P]</b> . <span> 外国专利：  CL2003001631A1 </span> <span> . 2004-09-03</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：将海洋细菌益生菌封装在藻酸盐基质和/或角叉菜胶中的活益生菌菌株的封装方法可长期存活，PERmite使它们易于使用。 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704028287876','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" 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