Amberlite IRA 900 versus calcium alginate in immobilization of a novel, engineered β‐fructofuranosidase for short‐chain fructooligosaccharide synthesis from sucrose

Bedzo Oscar K. K.; Trollope Kim; Gottumukkala Lalitha D.; Coetzee Gerhardt; G?rgens Johann F.

摘要

Abstract > The immobilization of β‐fructofuranosidase for short‐chain fructooligosaccharide (scFOS) synthesis holds the potential for a more efficient use of the biocatalyst. However, the choice of carrier and immobilization technique is a key to achieving that efficiency. In this study, calcium alginate (CA), Amberlite IRA 900 (AI900) and Dowex Marathon MSA (DMM) were tested as supports for immobilizing a novel engineered β‐fructofuranosidase from Aspergillus japonicus for scFOS synthesis. Several immobilization parameters were estimated to ascertain the effectiveness of the carriers in immobilizing the enzyme. The performance of the immobilized biocatalysts are compared in terms of the yield of scFOS produced and reusability. The selection of carriers and reagents was motivated by the need to ensure safety of application in the production of food‐grade products. The CA and AI900 both recorded impressive immobilization yields of 82 and 62%, respectively, while the DMM recorded 47%. Enzyme immobilizations on CA, AI900 and DMM showed activity recoveries of 23, 27, and 17%, respectively. The CA, AI900 immobilized and the free enzymes recorded their highest scFOS yields of 59, 53, and 61%, respectively. The AI900 immobilized enzyme produced a consistent scFOS yield and composition for 12 batch cycles but for the CA immobilized enzyme, only 6 batch cycles gave a consistent scFOS yield. In its first record of application in scFOS production, the AI900 anion exchange resin exhibited potential as an adequate carrier for industrial application with possible savings on cost of immobilization and reduced technical difficulty. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 用于短链果寡糖（SCFO）合成的β-果呋喃糖苷酶的固定含有更有效地使用生物催眠效果的可能性。然而，载体和固定技术的选择是实现该效率的关键。在本研究中，藻酸钙（CA），Amberlite IRA 900（AI900）和Dowex Marathon MSA（DOWEXMARATHMSA（DWM）被检测为用于固定新型工程化β-FructofuranOnosid酶的支持曲霉属japonicus </ i> 用于SCFOS合成。估计若干固定参数以确定载体在固定酶方面的有效性。在生产和可重复使用性的基础菌属的产量方面比较了固定化生物催化剂的性能。载体和试剂的选择是有必要确保在生产食品级产品的应用安全的情况下。 CA和AI900分别记录了82和62％的令人印象深刻的固定产量，而DMM记录47％。 Ca，Ai900和DMM上的酶固定筛查分别为23,27和17％的活性回收率。固定的CA，AI900，自由酶分别记录了59,53和61％的最高SCFO产率。 AI900固定化酶产生了一致的SCFO产率和12分批循环的组合物，但对于Ca固定化酶，只有6个分批循环得到一致的SCFO产率。在其在SCFOS生产中的第一次申请记录中，AI900阴离子交换树脂表现为适用于工业应用的适当载体，可能会节省固定的成本和减少技术难度。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第3期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bedzo Oscar K. K.&option=202" target="_blank" rel="nofollow">Bedzo Oscar K. K.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Trollope Kim&option=202" target="_blank" rel="nofollow">Trollope Kim;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gottumukkala Lalitha D.&option=202" target="_blank" rel="nofollow">Gottumukkala Lalitha D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Coetzee Gerhardt&option=202" target="_blank" rel="nofollow">Coetzee Gerhardt;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=G?rgens Johann F.&option=202" target="_blank" rel="nofollow">G?rgens Johann F.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Process EngineeringStellenbosch UniversityStellenbosch South Africa;</p> <p>Department of MicrobiologyStellenbosch UniversityStellenbosch South Africa;</p> <p>Department of Process EngineeringStellenbosch UniversityStellenbosch South Africa;</p> <p>Department of Process EngineeringStellenbosch UniversityStellenbosch South Africa;</p> <p>Department of Process EngineeringStellenbosch UniversityStellenbosch South Africa;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=adsorption&option=203" rel="nofollow">adsorption;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=entrapment&option=203" rel="nofollow">entrapment;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fructooligosaccharides&option=203" rel="nofollow">fructooligosaccharides;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=frustosyltransferase&option=203" rel="nofollow">frustosyltransferase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=immobilization&option=203" rel="nofollow">immobilization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ion exchange&option=203" rel="nofollow">ion exchange;</a> </p> <div class="translation"> 机译：吸附;夹带;果寡糖;弗罗粒基转移酶;固定化;离子交换; 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