Bioprocess optimization of laccase production through solid substrate fermentation using Perenniporia tephropora‐L168 and its application in bioremediation of triaryl‐methane dye

Patil Nayna D.; Chopade Lalit R.; Narkhede Ketan P.; Chaudhari Bhushan L.; Mahajan Raghunath T.

摘要

Abstract > Laccases are multi copper oxidases that can oxidize both phenolic and nonphenolic lignin related compounds. Consequently, there has been continuous demand for laccases for the oxidative degradation of phenolic dyes in effluents. In view of this, the present work was focused on laccase production by solid substrate fermentation using a newly isolated fungus Perenniporia tephropora ‐L168. To intensify the laccase production, the process parameters pH, nitrogen, inducer, and substrate: water ratio were optimized by using statistical model. A set of optimal conditions noted were pH 3, nitrogen 0.001?g/L; inducer 0.5% and substrate: water ratio (1:10), which yielded laccase 1,160?U/g. The crude laccase exhibited noteworthy potential to degrade a triaryl‐methane dye especially Malachite green. Also, during bioremediation studies, the statistical process optimization could achieve 81% decolourization within 180?min. The laccase treatment brought chemical transformation in malachite green as evident from UV–Visible spectra, FTIR, HPLC while toxicity against bacteria and fungi was also reduced. During phytotoxicity study, effect of treated and untreated dye on germination of seed was analyzed. Interestingly, the germination index for Vigna aconitifolia and Vigna radiata was increased by two and fourfold, respectively. Overall, this work demonstrates optimized production of laccase using Perenniporia tephropora ‐L168 and its efficient bioremediation potential for triaryl‐methane dye. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 漆酶是多铜氧化酶，其可以氧化酚类和非对酚木质素的木质素相关化合物。因此，对流出物中酚醛染料的氧化降解一直存在连续需求。鉴于此，目前的作品专注于使用新隔离的真菌的固体基质发酵的漆酶生产 perenniporia tephropora </ i> -1168。为了加强漆酶生产，通过使用统计模型优化工艺参数pH，氮气，诱导剂和衬底：水比。注意到一组最佳条件是pH 3，氮0.001Ω·克/升;诱导剂0.5％和底物：水比（1:10），其产生漆酶1,160. U / g。粗漆酶表现出有效的潜力，可降解三芳基甲烷染料，特别是孔雀石绿。此外，在生物修复研究期间，统计过程优化可以在180年内达到81％的脱色剂。从紫外线可见光谱，FTIR，HPLC的胃肠杆菌，HPLC的毒性，同时对细菌和真菌的毒性进行了显而易见，漆晶晶晶体治疗造成化学转化。在植物毒性研究期间，分析了治疗和未处理染料对种子萌发的影响。有趣的是，发芽指数豇豆aconitifolia </ i> 和豇豆radiata </ i> 分别增加了两个和四倍。总体而言，这项工作展示了使用的优化生产漆 perenniporia tephropora </ i> -1168及其高效的三芳基甲烷染料的生物修复潜力。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2020年第2期</span><b style="margin: 0 2px;">|</b><span>共12页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Patil Nayna D.&option=202" target="_blank" rel="nofollow">Patil Nayna D.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chopade Lalit R.&option=202" target="_blank" rel="nofollow">Chopade Lalit R.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Narkhede Ketan P.&option=202" target="_blank" rel="nofollow">Narkhede Ketan P.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chaudhari Bhushan L.&option=202" target="_blank" rel="nofollow">Chaudhari Bhushan L.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mahajan Raghunath T.&option=202" target="_blank" rel="nofollow">Mahajan Raghunath T.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of MicrobiologyMoolji Jaitha CollegeJalgaon Maharashtra India;</p> <p>Department of Microbiology School of Life SciencesKavayitri Bahinabai Chaudhari North Maharashtra UniversityJalgaon Maharashtra India;</p> <p>Department of MicrobiologyMoolji Jaitha CollegeJalgaon Maharashtra India;</p> <p>Department of Microbiology School of Life SciencesKavayitri Bahinabai Chaudhari North Maharashtra UniversityJalgaon Maharashtra India;</p> <p>Department of MicrobiologyMoolji Jaitha CollegeJalgaon Maharashtra India;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioremediation&option=203" rel="nofollow">bioremediation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=dye degradation&option=203" rel="nofollow">dye degradation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=laccase&option=203" rel="nofollow">laccase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=solid substrate fermentation&option=203" rel="nofollow">solid substrate fermentation;</a> </p> <div class="translation"> 机译：生物修复;染料降解;漆酶;固体基质发酵; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> 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Wen-Fu Xin</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Yu-Hong Wei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,Yu-Hong Wei</a> <span> <a href="/conference-cn-19543/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第五届全国化工年会 </a> <span> <span> . 2008</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130418781695.html">Triaryl methane dyes, and its applications</a> <b>[P]</b> . <span> 外国专利：  JP5549350B2 </span> <span> . 2014-07-16</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：三芳基甲烷染料及其应用 </span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06130494389070.html">Basic dyes of the series of triaryl - methane and their method of production.</a> <b>[P]</b> . <span> 外国专利：  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