A novel simple method for resolving overlapped spectral data with wider range of applicability

摘要

Smart simple spectrophotometric method was developed for simultaneous detemination of compunds with interfering spectra in binary mixtures without previous separation, showing significant advantages over the conventional methods regarding minimum data manipulation and applicability. The new method is based on a modification for the ratio subtraction and derivative ratio methods. This modification enabled wider range of application. The proposed method was applied for the determination of brimonidine and timolol in laboratory prepared mixtures with mean percentage recoveries 100.40 ± 2.29 and 101.23 ± 1.30 respectively, and in their pharmaceutical formulation with mean percentage recoveries 101.08 ±0.44 and 100.66±0.52 respectively. The suggested method was validated according to USP 2005 guidelines and can be applied for routine quality control testing.