Determination of (3aRS,4SR,7RS,7aSR)-2-(Tricyclo-[3.3.1.13,7] decan-1-yl)-4,5,6,7-tetrahydro-4,7-epoxyisoindoline-l, 3-dione (SU2162) in rat plasma by high-performance liquid chromatography with UV detection

摘要

A selective and sensitive high-performance liquid chromatography method has been developed and validated for determination of (3aRS,4SR,7RS,7aSR)-2-(Tricyclo-[3.3.1.13,7]decan-1-yl)- 4,5,6,7-tetrahydro-4,7- epoxyisoindoline-l,3-dione (SU2162) in rat plasma. The method of plasma disposal involved extraction and deproteinization by acetonitrile, dryness under a stream of nitrogen, and reconstituted with acetonitrile. Chromatographic separation was achieved on a reversed-phase C18 column using acetonitrile and water (40:60) as mobile phase at a flow rate of 1.0 ml/min. The assay was linear in the range of 0.200-16.00μg/ml with a correlation coefficient (r2) of 0.9955. Limits of detection and quantification were 0.204ug/ml and 0.816μg/ml, respectively. The precision and accuracy of the method were within the acceptable limits. The absolute recoveries of SU2162 in plasma were acceptable. The method was successfully applied to study the pharmacokinetics of SU2162 in rats.