Mobility Labeling for Parallel CID of Ion Mixtures

摘要

An ion mobility/mass spectrometry technique has been developed to record collision-induced dissociation pat-terns for multiple ions in a parallel fashion. In this approach, a mixture of ions is separated in a drift tube on the basis of differences in mobilities through a buffer gas. As the ions exit the drift tube, they are accelerated into a collision cell and the ensuing fragment ions are dispersed by differences in mass-to-charge (m/z) ratios in a time-of-ffight mass spectrometer. Fragment ions that are formed in the collision cell have drift limes that are comcident with their antecedent parent ions, allowing the origin of all fragments formed from the mixture of ions to be determined. The approach is demonstrated by examm-ing fragmentation patterns of the IM + H1~ parent and a series of a-, b-, andy-type fragments of In~AlaZ3]methionine enkephalin.