Detection and Separation of Gas-Phase carbon-Centered Radicals from Cigarette Smoke and Diesel Exhaust

摘要

Carbon-centered radicals were trapped from' gas-phase cigarette smoke and diesel engine exhaust by reaction with a nitroxide, 3-amino-2,5,5-tetramethyl-1-pyrrolidinyloxy (3AP). The resulting mixture of stable, diamagnetic adducts was derivatized with naphthalenedicarbox-aldehyde (NDA) to produce highly fluorescent products. Derivatives were separated by high-performance liquid chromatography (HPLC), which revealed distinctly different suites of radicals present in the two systems. Integration of HPLC peaks gave approximately 22± 7 nmol of radicals per cigarette and 3±1 nmol of radicals per liter of diesel engine exhaust. An estimated 8-10 different carbon-centered radical species are present in each system.