Scanning Optical Microscopy with an Electrogenerated Chemiluminescent Light Source at a Nanometer Tip

摘要

Electrogenerated chemiluminescence at electrodes with effective diameters down to 155 nm was used as a stable light source for near-field scanning optical microscopy imaging of an interdigitated array and a submicrometer size test substrate. Light was generated in a thin (～500 μm) layer of an aqueous solution of 15 mM Ru(bpy)_(3)~(2+) and 100 mM tri-n-propylamine in a pH 7.5 buffer. The resolution obtained was compared to that found with a micrometer size electrode. The shear force from the tip attached to a quartz tuning fork was used to monitor and control the tip-to-substrate separation within the near field regime.