Thermal shift assay to probe melting of thrombin, fibrinogen, fibrin monomer, and fibrin: Gly-Pro-Arg-Pro induces a fibrin monomer-like state in fibrinogen

摘要

Background: Thrombin activates fibrinogen and binds the fibrin E-domain (K-d similar to 2.8 mu M) and the splice variant gamma'-domain (K-d similar to 0.1 mu M). We investigated if the loading of D-Phe-Pro-Arg-chloromethylketone inhibited thrombin (PPACK-thrombin) onto fibrin could enhance fibrin stability.