Chip with Twin Anchors for Reduced Ion Suupression and Improved Mass Accuracy in MALDI-TOF Mass Spectrometry

摘要

A new sample target for matrix-assisted laser desorption/ionization mass spectrometry is described. The targetconsists of pairs of elevated hydrophilic anchor surfaces, positioned in proximity onto a microchip. The anchors areused to obtain separate preparations of sample and external standard, while both anchor surfaces are irradiated simultaneously by the laser pulse. Using a standard, based on six peptides, a 2-fold improvement in mass accuracy is observed. Also, ion suppression is significantly reduced. With a one peptide calibration standard, 22 tryptic fragments from a BSA digest are detected using the twin-anchor concept, whereas only 14 fragments are detected when the sample and standard are laser-ablated as a mixture from a conventional anchor target. A volume of ~30 pL of sample solution of angiotensin I is transferred to the anchor surface, under a thin layer of a perfluorocarbon, to prevent a concentration bias due to evaporation. With this arrangement, a detection limit of 1.5 amol was achieved with a signal-to-noise ratio of 22: 1.