An Efficient Liquid Chromatography-Mass Spectrometry Interface for the Generation of Electron Ionization Spectra

摘要

The use of a new LU—MS interface (cap-El), part of a Waters Integrity system, capable of generating El spectra at micro flow rates is presented. The cap-El interface relies on the production of a fine aerosol by means of a nebulizer and supported by a nitrogen jet. Sensitivity, response linearity, reproducibility, and LU compatibility of the interface were thoroughly examined using testoster-one, caffeine, a mixture of antiinflammatoiy drugs, and 3,4-dihydroxybeuzoic acid as test compounds. The inter-face is fully compatible with LU requirements such as high-water- and/or -buffer-content mobile phases. Repro-ducibility, high sensitivity in scan mode, as well, to produce library-searchable El spectra, 2 orders of mag-nitude linearity, together with an intrinsic simplicity of the entire system are the key features of cap-El interface.