A novel liquid chromatographic system which enables high temperature ultrafast liquid chromatography (HTU-FLC) has been designed through the careful consideration of heat transfer, band broadening, and pressure drop. Studies of the effect of linear velocity on the HETP show that column efficiency at high velocity, especially of well-retained solutes, dramatically improves at higher temperatures. At 150℃, at a flow rate of 15 mL/min with a 5 cm by 4.6 mm (i.d.) column packed with 3 μm polystyrene-coated zirconia porous particles, long chain alkylphenones were completely resolved, and the analysis time could be decreased by a factor of 50 compared to that at room temperature (25℃) at a conventional flow rate (4 mL/min). In addition, using pure water as the mobile phase, five phenols were separated in less than 30 s.
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机译：通过仔细考虑传热，谱带展宽和压降，设计了一种能够进行高温超快液相色谱（HTU-FLC）的新型液相色谱系统。对线速度对HETP的影响的研究表明，在高温下，尤其是保留良好的溶质，在高速下色谱柱的效率会大大提高。在150℃，流速为15 mL / min的条件下，用填充有3μm聚苯乙烯涂层氧化锆多孔颗粒的5 cm x 4.6 mm（id）色谱柱可以完全分辨长链烷基苯酮，并且可以减少分析时间与室温（25℃）和传统流速（4 mL / min）相比要高出50倍。此外，使用纯水作为流动相，可以在不到30秒的时间内分离出5种酚。
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