AMPEROMETRIC DETECTION OF PEROXIDES WITH POLY(ANILINOMETHYLFERROCENE)-MODIFIED ENZYME ELECTRODES

摘要

An amperometric enzyme electrode employing covalently attached horseradish peroxidase to glassy carbon electrode and a ferrocene-modified polyaniline film deposited by the electrochemical polymerization of N-(ferrocenylmethyl)aniline monomer is reported. The electrode responded rapidly to micromolar concentrations of peroxides, in accordance with the following trend: hydrogen peroxide > cumene hydroperoxide > tert-butyl hydroperoxide. The effect of operational parameters, such as methods of enzyme immobilization, operating potential of the working electrode, pH, and effect of molecular oxygen, were explored for optimum analytical performance. The dynamic properties of this enzyme electrode were exploited for the detection in now injection analysis. Applicability of the enzyme electrode for measurement of peroxide in real sample was demonstrated.