Monitoring D-Serine Dynamics in the Rat Brain Using Online Microdialysis-Capillary Electrophoresfs

摘要

D-Serine was detected in dialysate collected from the rat striatum using an online microdialysis-CE-LIF instrument.Dialysate can be analyzed every 12.5 s using the online instrument,giving much better temporal resolution than previously possible for D-serine.Basal concentrations of D-serine(8(+-)2 muM),glutamate(0.8(+-)0.2 muM),GABA(0.11(+-)0.04 muM),and L-serine(23(+-)4 muM)were measured.Increases in the concentrations of these neu-rochemicals induced by the introduction of high-K~+ aCSF were quantitated.Notably,an increase in D-serine concentration in response to high-K~+ aCSF was observed for the first time.The identity of the D-serine peak was confirmed unambiguously using D-amino acid oxidase to selectively remove D-serine from a dialysate sample.The microdialysis-CE-LIF instrument was able to monitor this enzymatic reaction as it proceeded over a period of 60 min,demonstrating that online microdialysis-CE-LIF is not only useful in monitoring in vivo dynamics but can also be used to monitor other chemical systems.