ISOLATION AND CHARACTERIZATION OF MICRO SATELLITE LOCI IN PRIMROSE (PRIMULA VULGARIS)

摘要

Microsatellite loci were developed tor rrimula vulgaris, a distylous, insect-pollinated, long-lived perennial herb. Primula vulgaris is rare and declining in Belgium. Eighteen microsatellite primer pairs were tested on 267 P. vulgaris individuals andon 246 individuals of its common congener Primula elatior to test for cross-species amplification. Only three polymorphic microsatellite loci amplified successfully in P. vulgaris and only two in P. elatior. Between 4 and 11 alleles were found per locusin P. vulgaris and 8-11 alleles per locus in P. elatior. Expected heterozygosity ranged from 0.496-0.620 and from 0.830-0.880 for P. vulgaris and P. elatior, respectively.