Surface-Plasmon-Enhanced Fluorescence Spectroscopy for DNA Detection Using Fluorescently Labeled PNA as DNA Indicator

摘要

A rapid detection of the presence/absence of a specific DNA sequence is crucial for the identification of genetically modified organisms (GMOs) in foods, the diagnosis of infections, and the monitoring of the environment. Most available methods rely on the immobilization of DNA probes onto sensor surfaces and their subsequent hybridization with the target DNA from the solution. Recently, several researchers demonstrated that it is possible to use conjugated polymers[1] or DNA-functionalized nanoparticles[2] for homogeneous DNA detection, thus avoiding the difficulties associated with DNA-probe immobilization. However, the proposed strategies involve several process steps, which makes them unsuitable for practical DNA detection.