Optimisation of extraction methods and quantification of benzo[ a]pyrene and benz[ a]anthracene in yerba maté tea by isotope dilution mass spectrometry

摘要

AbstractA gas chromatography–isotope dilution mass spectrometry (GC–IDMS) technique was developed for the quantification of two heavy polyaromatic hydrocarbons (PAHs), benz[a]anthracene and benzo[a]pyrene, in yerba maté tea (maté). The optimisation of two extraction methods, namely liquid–liquid extraction and accelerated solvent extraction, was carried out. Both optimised methods were validated using a certified reference material of fine dust and the results were within the expanded uncertainties at 95% confidence level. Recoveries of 99.2–106.7% with RSD of measurements of 1.1–2.3% were achieved for benz[a]anthracene. Recoveries of 95.7–101.9% with RSD of measurements of 0.4–1.4% were achieved for benzo[a]pyrene. The validated methods were applied for the extraction of benz[a]anthracene and benzo[a]pyrene in maté powder from NIST. A metrological approach was undertaken to ensure the traceability of measurement results. The uncertainties associated with the results were rigorously evaluated and also reported herein.

Graphical abstractQuantification of benz[a]anthracene and benzo[a]pyrene using IDMS

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