Coupling of the ring-oven-based preconcentration technique and surface-enhanced Raman spectroscopy: Application for the determination of purine bases in DNA

摘要

AbstractIn this paper, we present the advantages and limitations of the coupling of a ring-oven-based preconcentration technique and surface-enhanced Raman spectroscopy (SERS). Three different methods to promote analyte adsorption on gold nanoparticles using crystal violet as a probe molecule were assessed. The results showed significant improvements in sampling process, selectivity, sensitivity, repeatability (less than?±?10%), and detection limits (nanomolar level) using a sample volume as small as 300?μL. Finally, the standard addition method was successfully applied to the quantitative SERS detection of adenine and guanine in calf thymus DNA after ring-oven preconcentration with a calculated value of (G?+?C)/(A?+?T) close to the literature value. This work could therefore pave the way to quantifying a wide variety of biologically relevant compounds in real-world samples via the use of a biodegradable, low-cost and disposable paper platform for SERS.Graphical abstractDisplay OmittedHighlights?Synergistic coupling of the ring-oven preconcentration and SERS for improving sampling process.?Ring-oven preconcentration allows the in situ synthesis of gold nanoparticles on filter paper.?The proposed system provides a large and tunable sensibility.?Individual SERS detection of DNA bases was performed at nanomolar level.?Adenine and guanine were quantified in DNA using internal standards and a standard addition method.]]>