Dual-recognition surface-enhanced Raman scattering(SERS)biosensor for pathogenic bacteria detection by using vancomycin-SERS tags and aptamer-Fe3O4@Au

摘要

Rapid and reliable detection of pathogenic bacteria is vital to prevent and control bacterial diseases. In this study, we present a magnetically assisted surface-enhanced Raman scattering (SERS) biosensor based on the dual-recognition of bacterial cell by aptamer and antibiotic molecules. Aptamer-Fe3O4@Au magnetic nanoparticles (AuMNPs) were synthesized as magnetic and SERS activated substrate for specific bacteria enrichment, vancomycin-SERS tags (Au@MBA) were prepared for the sensitive quantification of pathogenic bacteria. Due to the Au-shell based dual-SERS enhancement and aptamer/vancomycin based dual-recognition ability, a detection limit of 3 cells/mL with a wide dynamic linear range from 10 to 10(7) cells/mL can be achieved within 50 min without other non-target bacteria interference. When applied in real samples, the approach shows recoveries from 95.0% to 106.4% with relative standard derivation (RSD) less than 5.3%. The SERS strategy could be used to detect a broad range of bacteria by using different aptamers, moreover, the simple operation and precise quantification ability empower this assay great potential in the application of food safety and infectious disease point-of-care diagnosis. (C) 2019 Elsevier B.V. All rights reserved.