Optically Transparent Thin-Film Electrode Chip for Spectroelectrochemical Sensing

摘要

A novel microfabricated optically transparent thin-film electrode chip for fluorescence and absorption spectroelectrochemistry has been developed. The working electrode was composed of indium tin oxide (ITO); the quasi-reference and auxiliary electrodes were composed of platinum. The stability of the platinum quasi-reference electrode was improved by coating it with a planar, solid state Ag/AgCl layer. The Ag/AgCl reference was characterized with scanning electron microscopy and energy-dispersive X-ray spectroscopy. Cyclic voltammetry measurements showed that the electrode chip was comparable to a standard electrochemical cell. Randles-Sevcik analysis of 10 mM K-3[Fe(CN)(6)] in 0.1 M KCl using the electrode chip gave a diffusion coefficient of 1.59 x 10(-6) cm(2)/s, in comparison to the value of 2.38 x 10(6) cm(2)/s using a standard electrochemical cell. By using the electrode chip in an optically transparent thin-layer electrode (OTTLE), the absorption based spectroelectrochemical modulation of [Fe(CN)(6)](3/4) was demonstrated, as well as the fluorescence based modulation of [Ru(bpy)(3)](2+/3+). For the fluorescence spectroelectrochemical determination of [Ru(bpy)(3)](2+), a detection limit of 36 nM was observed.