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Enhanced Imaging of Specific Cell-Surface Glycosylation Based on Multi-FRET

机译:基于多重尺寸的特定细胞表面糖基化的增强成像

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摘要

Cell-surface glycosylation contains abundant biological information that reflects cell physiological state, and it is of great value to image cell-surface glycosylation to elucidate its functions. Here we present a hybridization chain reaction (HCR)-based multifluorescence resonance energy transfer (multi-FRET) method for specific imaging of cell-surface glycosylation. By installing donors through metabolic glycan labeling and acceptors through aptamer-tethered nanoassemblies on the same glycoconjugate, intramolecular multi-FRET occurs due to near donor–acceptor distance. Benefiting from amplified effect and spatial flexibility of the HCR nanoassemblies, enhanced multi-FRET imaging of specific cell-surface glycosylation can be obtained. With this HCR-based multi-FRET method, we achieved obvious contrast in imaging of protein-specific GalNAcylation on 7211 cell surfaces. In addition, we demonstrated the general applicability of this method by visualizing the protein-specific sialylation on CEM cell surfaces. Furthermore, the expression changes of CEM cell-surface protein-specific sialylation under drug treatment was accurately monitored. This developed imaging method may provide a powerful tool in researching glycosylation functions, discovering biomarkers, and screening drugs.
机译:细胞表面糖基化含有丰富的生物信息,反映细胞生理状态,并且对图像细胞表面糖基化具有很大的值,以阐明其功能。在这里,我们提出了一种杂交链反应(HCR)基础的多氟化浓缩能量转移(多重尺寸)方法,用于细胞表面糖基化的特异性成像。通过在相同的糖缀合物上通过适应性甘露甘露糖纳米粘合剂通过代谢聚糖标记和受护者安装捐赠者,由于靠近供体 - 受体距离而发生分子内的多尺寸。受益于HCR纳米组织的扩增效果和空间柔韧性,可以获得特定细胞表面糖基化的增强的多重尺寸成像。利用这种基于HCR的多重尺寸方法,我们在7211细胞表面上成像在蛋白质特异性加仑淀粉化的成像对比度。此外,我们通过在CEM细胞表面上可视化蛋白质特异性唾液化来证明了这种方法的一般适用性。此外,精确地监测了药物处理下CEM细胞表面蛋白质特异性唾液酸化的表达变化。这种发育的成像方法可以提供研究糖基化功能,发现生物标志物和筛选药物的强大工具。

著录项

  • 来源
    《Analytical chemistry》 |2018年第10期|共7页
  • 作者单位

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Hunan University Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Changsha 410082 China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分析化学;
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