NMR Spectroscopic Detection of Protein Protons and Longitudinal Relaxation Rates between 0.01 and 50 MHz

摘要

Nuclear magnetic relaxation data of water nuclei at variable fields provide valuable information on the dynamics of water-solute interactions.However,information can be collected only within certain field ranges in which the nuclear relaxation rates are field-dependent owing to the dispersion of the spectral density,J(omega,tau).The dispersion depends on the type of motion and on the observed nucleus.The most informative ~1H NMR spectroscopic frequency range for rotational motions is centered around 10 MHz for small proteins(M_w approx= 10~4 Da)and smaller frequencies for larger proteins and protein aggregates.