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Quantification of Drug Molecules in Live Single Cells Using the Single-Probe Mass Spectrometry Technique

机译:使用单探针质谱技术定量活单细胞中药物分子

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Analyzing cellular constituents on the single-cell level through mass spectrometry (MS) allows for a wide range of compounds to be studied simultaneously. However, there is a need for quantitative single-cell mass spectrometry (qSCMS) methods to fully characterize drug efficacy from individual cells within cell populations. In this study, qSCMS experiments were carried out using the Single-probe MS technique. The method was successfully used to perform rapid absolute quantifications of the anticancer drug irinotecan in individual mammalian cancer cells under ambient conditions in real time. Traditional liquid chromatography/mass spectrometry (LC/MS) quantifications of irinotecan in cell lysate samples were used to compare the results from Single-probe qSCMS. This technique showcases heterogeneity of drug efficacy on the single-cell level.
机译:通过质谱(MS)分析单细胞水平上的细胞成分允许同时研究各种化合物。 然而,需要定量单细胞质谱(QSCMS)方法,以完全表征来自细胞群中的个体细胞的药物效果。 在该研究中,使用单探针MS技术进行QSCMS实验。 该方法成功地用于实时在环境条件下在各个哺乳动物癌细胞中的抗癌药物伊替康的快速定量。 使用传统的液相色谱/质谱(LC / MS)细胞裂解物样品中的伊替替康的量化来比较单探针QSCM的结果。 该技术展示了对单细胞水平的药物效能的异质性。

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