Supercritical-Fluid Chromatography On-Chip with Two-Photon-Excited-Fluorescence Detection for High-Speed Chiral Separations

摘要

Herein, we present the first example of microchip-based supercritical-fluid chromatography (SFC). A microfluidic-glass-chip platform with pressure and temperature control for fast and efficient on column injection is described. This enabled fast and efficient separation of chiral and achiral compounds within seconds and also employed two-photon-excitated-fluorescence detection. Peak shapes were highly regular and symmetric even for linear flow rates over the packed microchip column in a range of up to 20 mm.s(-1).